Paul Rosenberg, M.D., Ph.D.

 

Associate Professor of Neurology

Boston Children's Hospital
Center for Life Science, Room 13-073
3 Blackfan Circle
Boston, MA 02115
Tel: 617-919-2634
Fax: 617-919-2380
Email: paul.rosenberg@childrens.harvard.edu
Visit my lab page here.




We are studying the function, regulation, and expression of glutamate transporters in the brain. Glutamate transport is important in the physiology of excitatory synapses and for the survival of neurons and oligodendrocytes because of the phenomenon of excitotoxicity. We recently demonstrated that the glutamate transporter GLT1 is the major glutamate transporter in excitatory terminals, and now we are interested in determining the contribution of presynaptic glutamate transport to the function of excitatory synapses and to the pathophysiology of neurodegenerative diseases, in particular, Huntington’s disease. Approaches include yeast 2-hybrid studies seeking protein interactors, patch-clamp recording of glutamate currents from neurons, astrocytes, and cell lines expressing glutamate transporters, to characterize the biophysical properties of glutamate transporters, and knockouts to investigate the role of presynaptic GLT1 expression in learning and memory and excitotoxic injury.

In the second area of interest, we are studying the molecular mechanisms by which oxidative stress and excitotoxicity produce cell death in neurons and oligodendrocytes, and the role of these mechanisms in the pathogenesis of neuron and oligodendrocyte injury in cerebral palsy, stroke, Huntington’s disease, and amyotrophic lateral sclerosis. Tissue culture models of oxidative stress-, excitotoxin-, and cytokine-evoked cell death are currently under investigation.

Finally, another project is focused on the mechanisms underlying the control of behavioral state. We are particularly interested in the roles of nitric oxide and adenosine. We have found that the response to sleep deprivation (recovery sleep) is due to the production of nitric oxide by inducible nitric oxide synthase in the basal forebrain. The major questions emerging from this work include: which cells express iNOS, what activates iNOS expression in these cells, what is the molecular pathway activated by release of nitric oxide and in what cells does the activation occur, and how is REM recovery sleep regulated?


For a complete listing of publications click here.



Last Update: 11/7/2013