Joslin Diabetes Center
One Joslin Place
Boston, MA 02215
Our research focuses on two distinct adult stem cell populations: hematopoietic stem cells (HSC), which constantly maintain and can fully regenerate the entire blood system, and skeletal muscle satellite cells, which analogously mediate both homeostatic and regenerative skeletal muscle formation. These studies rely heavily on the prospective isolation of rare subsets of tissue-specific precursor cells, and the evaluation of their function in vivo in appropriate mouse models. The ultimate goals of this work will be to define both intrinsic and extrinsic factors that influence the migration, expansion, and survival of blood-forming and muscle-forming stem cells, to map cell lineage relationships in their differentiation to generate mature progeny cells, and to evaluate their therapeutic potential in response to tissue damage and disease.
Ongoing projects include:
Evaluation of the role of the transcription factor early growth response-1 (Egr-1) in HSC expansion and migration
Analysis of changes in the HSC microenvironment that accompany HSC mobilization, aging and leukemic transformation
Determination of the in vitro and in vivo self-renewal and differentiation potentials of prospectively isolated muscle stem cells
1. Min IM, Pietramaggiori G, Kim FS, Passegue E, Stevenson KE, Wagers AJ. The transcription factor EGR1 controls both the proliferation and localization of hematopoietic stem cells. Cell Stem Cell 2008;2:380-391.
2. Cerletti M, Jurga S, Witczak CA, Hirshman MF, Shadrach JL, Goodyear LJ, Wagers AJ. Highly efficient, functional engraftment of skeletal muscle stem cells in dystrophic muscles. Cell 2008;134:37-47.
3. Forsberg EC*, Passegue E*, Prohaska SS*, Wagers AJ*, Koeva M, Stuart JM, Weissman IL. Molecular signatures of quiescent, mobilized and leukemia-initiating hematopoietic stem cells. PLoS One 2010 Jan 20;5(1):e8785.
4. Hettmer S, Wagers AJ. Muscling in: Uncovering the origins of rhabdomyosarcoma. Nat. Med. 2010;16:171-3.
5. Young AP, Wagers AJ. Pax3 induces differentiation of juvenile skeletal muscle stem cells without transcriptional upregulation of canonical myogenic regulatory factors. J. Cell Sci., in press.
Last Update: 1/6/2014