MGH, Division of Infectious Disease
55 Fruit Street
Boston, MA 02115
Lab Members: 3 Postdoctoral Fellows, 2 Technicians, 1 Graduate Student, 1 Undergraduate Student
Jatin M. Vyas received his PhD in Immunology working in the laboratory of Robert Rich at the Baylor College of Medicine. After completing his MD at Baylor, he performed his internship and medical residency at the Massachusetts General Hospital. He completed his Infectious Disease Fellowship at the MGH/BWH. He did his post-doctoral work in the laboratory of Hidde Ploegh.
The Vyas laboratory at the MGH focuses on the host response to fungal pathogens. Dendritic cells (DCs) represent the bridge between innate and adaptive immunity. This cell type possesses the unique capacity to activate naïve T cells. In order to carry out this function, DCs capture microorganisms and place them in membrane-delimited compartments termed phagosomes. We use fungal pathogens of clinical importance including Cryptococcus neoformans, Aspergillus fumigatus and Candida albicans as the model pathogens to study phagosome formation and maturation. Using live cell imaging with spinning disk confocal microscopy, we visualize primary DCs and its responses to exposure to fungi in real time using fluorescently-tagged proteins of the endocytic pathway and the antigen processing and presentation machinery. We examine the role of Dectin-1, LC-3 (member of the autophagy family of proteins) Toll-like receptors and members of the tetraspanin family and their effects on phagosomal maturation.
Kasperkovitz PV, Cardenas ML, Vyas JM. TLR9 is Actively Recruited to Aspergillus fumigatus Phagosomes and Requires the N-terminal Proteolytic Cleavage Domain for Proper Intracellular Trafficking. 2010. The Journal of Immunology. 185(12):7614-7622. PMCID: In process
Tam JM, Castro CE, Heath RJ, Cardenas ML, Xavier RJ, Lang MJ and Vyas JM. Control and Manipulation of Pathogens with an Optical Trap for Live Cell Imaging of Intercellular Interactions. 2010. PLoS One. 5(12):e15215. PMCID: PMC3013098
Artavanis-Tsakonas K, Kasperkovitz PV, Papa E, Cardenas ML, Khan NS, Van der Veen AG, Ploegh HL, Vyas JM. The Tetraspanin CD82 is Specifically Recruited to Fungal and Bacterial Phagosomes Prior to Acidification. 2011. Infection and Immunity. Mar;79(3) 1369-76.
Tam JM, Mansour MK, Khan NS, Yoder NC, Vyas JM. Use of fungal derived polysaccharide-conjugated particles to probe Dectin-1 responses in innate immunity. 2012. Integrative Biology (Camb). Feb 1;4(2) 220-7.
Mansour MK, Tam JM, Khan NS, Seward M, Davids PJ, Puranam S, Sokolovska A, Sykes DB, Dagher Z, Becker C, Tanne A, Reedy JL, Stuart LM, and Vyas JM. Dectin-1 activation controls maturation of β-1,3-glucan-containing phagosomes. 2013. Journal of Biological Chemistry. Apr 22. 288(22):16043-54
For a complete listing of publications click here.
Last Update: 9/16/2013