Immunology Faculty Member - Hidde Ploegh

Hidde Ploegh

Boston Children's Hospital
Karp Building, Room 06215
1 Blackfan Circle
Boston, MA 02115
Tel: 617-919-1613
Email: hidde.ploegh@childrens.harvard.edu



The Ploegh lab studies molecular aspects of immune recognition, using chemical, cell biological and biochemical approaches, complemented by appropriate in vivo models. We are particularly interested in the use of nanobodies (single domain antibody fragments derived from camelid heavy chain-only immunoglobulins) for non-invasive imaging by positron emission tomography. This has made it possible, for the first time, to track immune responses non-invasively, as shown for tumors in response to checkpoint blockade, next to be extended to infectious disease and autoimmunity. These same nanobodies can be modified with cytokines to re-engineer the tumor micro-environment and create more effective forms of immunotherapy. We are using Cas9/CRISPR methods to modify immunoglobulin germ line genes with sequence tags that enable site-specific modification of B cell receptors to visualize and track their behavior. These tools provide unique new models to study aspects of B cell activation that have so far defied a straight-forward analysis. Finally, the lab maintains an active interest in protein quality control, with particular emphasis on the ubiquitin-proteasome system.



Last Update: 5/1/2017



Publications



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