BBS Faculty Member - Konrad Hochedlinger

Konrad Hochedlinger

Department of Stem Cell and Regenerative Biology
Howard Hughes Medical Institute
Harvard Stem Cell Institute

Massachusetts General Hospital
Richard B. Simches Research Building, CPZN 4242
185 Cambridge Street
Boston, MA 02114
Tel: 617-643-2075
Fax: 617-643-3170
Email: khochedlinger@helix.mgh.harvard.edu
Visit my lab page here.



Our lab tries to understand the molecular mechanisms underlying pluripotency and nuclear reprogramming. Pluripotency denotes the ability of cells, such as embryonic stem (ES) cells, to give rise to all cell types of the mammalian body, while nuclear reprogramming is the dedifferentiation of a specialized cell back into a pluripotent state. Reprogramming does not normally occur in vivo but can be achieved experimentally by nuclear transfer, ES cell-somatic cell fusion and by directly inducing embryonic genes in somatic cells, generating so-called induced pluripotent (iPS) stem cells.

We are studying these processes functionally by establishing transgenic and knock-out mice and by manipulating murine and human ES cells. Combined with genome-wide approaches including RNAi and chemical screening we aim to dissect the mechanisms of pluripotency and epigenetic reprogramming. Ultimately, we hope that our research will aid in attempts to generate custom-tailored cells for treating and understanding disease.

Lab Members:

Sihem Cheloufi, PhD, Postdoctoral Associate
Benjamin Schwarz, MD/PhD, Postdoctoral Fellow
Ryan Michael Walsh, PhD Student (BBS)
Abby Sarkar, PhD Student, (BBS)
Jiho Choi, PhD Student, (BBS)
Marti Borkent, Visiting PhD Student (Erasmus University, Rotterdam)
Aaron Huebner, PhD, Postdoctoral Fellow
Justin Brumbaugh, PhD, Postdoctoral Fellow
Kyle Flaherty, Undergraduate Student (Harvard)
Caitlin Murphy, Administrative Assistant



Last Update: 6/19/2014



Publications

For a complete listing of publications click here.

 


 

Arnold K, Sarkar A, Yram MA, Polo JM, Bronson R, Sengupta S, Seandel M, Geijsen N, Hochedlinger K. Sox2(+) adult stem and progenitor cells are important for tissue regeneration and survival of mice. Cell Stem Cell. 2011 Oct 4;9(4):317-29. PMID: 21982232 PMCID: PMC3538360.

Stadtfeld, M., Apostolou, E., Ferrari, F., Choi, J., Walsh, R.M., Chen, T., Ooi, S.S., Kim, S.Y., Bestor, T.H., Shioda, T., Park, PJ, Hochedlinger, K. Ascorbic acid prevents loss of Dlk1-Dio3 imprinting and facilitates generation of all-iPS cell mice from terminally differentiated B cells. Nat Genet. 2012, 44, 398-405, S391-392. PMID: 22387999 PMCID: PMC3538378.

Polo JM, Anderssen E, Walsh RM, Schwarz BA, Nefzger CM, Lim SM, Borkent M, Apostolou E, Alaei S, Cloutier J, Bar-Nur O, Cheloufi S, Stadtfeld M, Figueroa ME, Robinton D, Natesan S, Melnick A, Zhu J, Ramaswamy S, Hochedlinger K. A Molecular Roadmap of Reprogramming Somatic Cells into iPS Cells. Cell. 2012 Dec 21 151 (7) 1617-32 doi: 10.1016/j.cell.2012.11.039. PMID: 23260147 PMCID: PMC3608203.

Apostolou E, Ferrari F, Walsh RM, Bar-Nur O, Stadtfeld M, Cheloufi S, Stuart HT, Polo JM, Ohsumi TK, Borowsky ML, Kharchenko PV, Park PJ, Hochedlinger K. Genome-wide chromatin interactions of the Nanog locus in pluripotency, differentiation, and reprogramming. Cell Stem Cell. 2013 Jun 6;12(6):699-712. doi:10.1016/j,stem,2013.04.013. Epub 2013 May 9. PMID: 23665121.

Apostolou E, Hochedlinger K. Chromatin dynamics during cellular reprogramming. Nature. 2013 Oct 24;502(7472):462-71. Review PMID: 24153299.



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